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Fully Automated Instrumentation for Analysis in C
elegans

by: Rock Pulak, Peter
Kalutkiewicz, Anthony
Ferrante, Greg
O'Connor, Jennifer Kean, Ralph
Clover

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International C. elegans Meeting (2001)  Key:
citeulike:3300505

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Abstract

The completion of the genomic sequencing of C. elegans and D. melanogaster
enormously advances the understanding of these model organisms. Both organisms
are ideally suited for broad system-wide experiments, like transcriptional
profiling using DNA microarrays, as well as gene function analysis and their
use in chemical screening for drug discovery. However, certain types of
experiments would benefit from the development of technologies for high-speed
automation and sensitive instrumentation. We have developed the COPAS BIOSORT
instrument which can accurately select and dispense user-specified numbers of
C. elegans at specific stages, ranging from embryo through adult, into
multiwell microtiter plates. In addition the COPAS BIOSORT is coupled to a
robotic arm for microtiter plate handling (Zymark Twister), allowing for the
analysis of very large numbers of populations. The contents of each well can
be analyzed at a later time for changes in developmental stage or gene
expression, with the use of COPAS ReFLX , an add-on module to the COPAS
BIOSORT . Analysis is based on the optical properties of the animals. Besides
detecting the natural light-scattering properties of C. elegans , the COPAS
BIOSORT can also be used with transgenic animals expressing jellyfish- or reef
coral-fluorescent proteins from worm promoters. The system simultaneously
performs a quantitative size, autofluorescence, and reporter fluorescence
analysis on each organism within a population contained in an individual well
(for example, 20 organisms per well) in less than 20 seconds. The data are
displayed, collected, analyzed, and stored for later retrieval. This
instrumentation has broad applications for genetic screening (for mutants) and
chemical screening (for drugs) that affect an optical phenotype. The
instrument can also be used for more specific applications such as identifying
chromosomal integrants and live-dead screening assays. We will present data
from several reconstruction experiments using the COPAS BIOSORT
instrumentation.

C. elegans / WormBase's tags for this article

c_elegans
caenorhabditis_elegans celegans
elegans meeting_abstract nematode
wormbase

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